Methods using different techniques of collagenase digestion and discontinued density gradient centrifugation , the adult swine and rat islets were prepared 方法采用不同的胶原酶消化法及不连续密度梯度纯化法。
The fetal liver stem cells were isolated by collagenase digestion , gravity sedimentation and density gradient centrifugation , identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition 采用胶原酶消化、重力沉降及密度梯度离心方法分离人胎肝干细胞,通过免疫细胞化学方法对其进行初步鉴定,以及应用流式细胞仪等对其生长状况进行评估。